Journal article
Sugarcane polyphenol and fiber to affect production of short-chain fatty acids and microbiota composition using in vitro digestion and pig faecal fermentation model
YT Loo, K Howell, H Suleria, P Zhang, C Gu, K Ng
Food Chemistry | Published : 2022
Abstract
This study aimed to examine the effects of sugarcane polyphenol and fiber (Phytolin + Fiber) on gut microbiota, short-chain fatty acids (SCFAs) production and phenolic metabolites production using in vitro digestion and fermentation model. Microbial profiling by 16S rRNA sequencing was used to analyze the pig faecal microbiota profile. SCFAs were identified and quantified by GC-FID, and phenolic metabolites were characterized by LC-ESI-QTOF-MS/MS. The results showed that Phytolin + Fiber exert synergistic effects on the pig gut microbiota by increasing the relative abundances of Lactobacillus and Catenibacterium, and decreasing the relative abundances of Mogibacterium, Dialister, and Escheri..
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Grants
Awarded by Métropole Toulon-Provence-Méditerranée
Funding Acknowledgements
This research is partially funded by The Product Maker (Australia) Pty Ltd (TPM) (Keysborough, Victoria, 3173, Australia) (University of Melbourne Agree ID 38900) which also provided the Phytolin and sugarcane fiber samples for the research. The authors would also like to acknowledge Dr Barry Kitchen (Chief Scientific Officer, Head Bioactives Division, TPC) for interesting and insightful comments regarding the work. YTL is a recipient of a Melbourne Research Scholarship.